Production of vaccine
Production of vaccine (expressing foreign proteins) in plants through integration of foreign genes into the plant genome of is a multistep procedure. As the first step, suitable antigene should be identified and isolated (amplified). The gene encoded for the selected antigenic protein should have ability to stimulate production of serum or mucosal antibodies in host body against the chosen pathogen interested. Next, the gene code of the selected antigenic protein has to be inserted in to a plant expression vector. The vector should contain a strong constitutive promoter which having ability to control expression of antigen protein within plant as well as selectable marker gene which is important for selection of transgenic plants.